寨卡登革 熱基孔肯雅熱三合一核·酸·檢測試劑（德國Genekam原裝進(jìn)口）

貨號：RF342

規(guī)格：100人份/盒

Principle and use:

This amplification kit has been manufactured by Genekam Biotechnology AG, Germany to detect different viruses: Chikungunya virus, Dengue virus (1-4) and Zika virus in real time PCR assay as multiplex. It is an absolute quantification. It detects viruses and indicates the load of each virus in unknown samples.  These are mosquito borne viruses, which can cause a major threat through different ways e.g. blood transfusion as well as organ transplantation to the donors along with creating normal disease profile in human population through mosquito bites. PCR is the method of choice to detect the viruses against serology methods like ELISA and Rapid tests as they cross reacts with each other.

Warning: Multiplex assays may have less sensitivity than simplex assay in some settings. Such multiplex assays have this technological disadvantage, but their advantages are economical, fast results and multiple parameters for screening, hence they should be used for fast screening e.g. blood banks to give answer whether a particular pathogen is present in unknown samples or not.

Real time PCR is based on fluorogenic dyes. In this kit, there are 4 probes / dyes, hence you have to program them in your machine, each probe indicates the presence of one specific virus:

First Probe: they are 6-Carboxy tetramethyl rhodamine (quencher) and Carboxy-fluorescein (reporter). Up to 40 Ct should be taken positive. Value between 40-45 Ct should be taken as marginal positive (doubtful). It indicates the presence of dengue virus 1 - 3.

Second probe: they are 6-Carboxy tetramethyl rhodamine (quencher) and HEX (reporter, it is available as VIC in some machines). Up to 40 Ct should be taken positive. Value between 40-45 Ct should be taken as marginal positive (doubtful). It indicates the presence of Zika virus.

Third probe: they are 6-Carboxy tetramethyl rhodamine (quencher) and ROX (reporter, it is available as ROX in some machines). Up to 40 Ct should be taken positive. Value between 40-45 Ct should be taken as marginal positive (doubtful). It indicates the presence of Chikungunya virus.

Fourth probe: they are 6-Carboxy tetramethyl rhodamine (quencher) and Cy5 (reporter, it is available as Cy5 in some machines). Up to 40 Ct should be taken positive. Value between 40-45 Ct should be taken as marginal positive (doubtful). It indicates the presence of dengue virus 2 - 4.

Hint: Please make sure that you have realtime machine, which can detect all 4 probes in their corresponding channels. User has to select above said setting in the software of the realtime machine.

This kit needs RNA which can be isolated from  blood, urine, mosquitoes, seminal samples, liver biopsies, serum, plasma, tissue, cell cultures and any body fluid. Kindly use good methods to isolate the RNA.

Safety precautions should be taken as these viruses are infectious for human beings. Always clean your hands before the test use and clean the hands after the test. Wash your face after the test, if possible. Disinfect your working place.

IMPORTANT: we added cotton or sponge in the lid of container of the kit, to avoid damage during transportation. Please remove this cotton or sponge from the lid of each container before storage.

Composition:

It contains the following (WARNING! THAW THE TUBES SLOWLY: NEVER THAW IN HEATING BLOCK OR WITH HEAT FROM HAND):

? Tube A (2 tubes)

? Tube B (2 tubes)

? Tube Y (1 tube)

? Positive (+Ve) Control (tube D1) (1 tube). This tube must be stored at -20°C.

? Negative (-Ve) Control (tube D2) (1 tube)

Please check them before you start. Please store them at -20°C and dark.

Equipment needed:

? Laboratory centrifuge

? microtubes (0.2ml)

? Pipette-tips with and without filter (10-100μl & 1-10μl)

? Pipettes (quality pipettes)

? Paper

? Pen

? Vortexer

? 96 well microplates for PCR

? Real time machine

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